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Estimation of apparent Na+ binding affinity with WT PomA/B and PomB mutants. The swimming speeds of cells (WT; wild-type PomA/B, Ala; C31A, Thr; C31T, Gly; C31G, Met; C31M, Val; C31V, Ile; C31I, Ser; C31S) were plotted against NaCl concentrations. Data were fit by the Michaelis-Menten equation.
