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. 2009 Jun 12;5:45–52. doi: 10.2142/biophysics.5.45

Figure 5.

Figure 5

(a) Swarms of RP6894 (ΔmotAB) cells harboring plasmids that express MotA/B(A39V) (A39V), MotA(M206G)/B(A39V) (A39V/M206G), MotA(M206A)/B(A39V) (A39V/M206A), MotA(M206S)/B(A39V) (A39V/M206S) or MotA(M206T)/B(A39V) (A39V/M206T). Transformants were cultured in liquid medium overnight, and aliquots were spotted onto a plate containing TG and 0.26% agar. Plates were incubated in the presence of 0.04% arabinose at 30°C for 20 hr. Vec. means a pBAD24 plasmid vector. (b) Western blotting analysis of MotA (left panel) and MotB (right panel) in whole cell extracts. Proteins were detected with an anti-MotA266 antibody and an anti-MotB2 antibody. (c) Swarms of NMB191 (ΔpomAB) cells harboring a plasmid that express PomA/B (WT), PomA/B(C31A) (C31A), PomA/B(L28A/C31A) (L28A/C31A) or PomA(L183F)/B(L28A/C31A) (L28A/C31A/L183F). Transformants were cultured in liquid medium overnight, and aliquots were spotted onto a plate containing VPG10 (1% polypeptone, 0.4% K2HPO4, 10 mM NaCl, 490 mM KCl, 0.5% glycerol) and 0.25% agar. Plates were incubated in the presence of 0.04% arabinose and 2.5 μg/ml chloramphenicol at 30°C for 12 hr. Vec. means a pBAD33 plasmid vector. (d) Western blotting analysis of PomA (left panel) and PomB (right panel) in whole cell extracts. Proteins were detected with an anti-PomA1312 antibody and an anti-PomB93 antibody.