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Twists and buckling of actin filaments observed in vitro. (A) Right-handed super-coiled actin filament. (B) Buckled actin filament. (C) Left-handed super-coiled actin filament. PE and BE represent the pointed and barbed ends of the actin filament, respectively. The pointed end of the actin filament is sticks by chance to a glass surface or is attached to the glass surface as illustrated.
