Example of a HDC-TEM application. (a) HDC-TEM image of ice-embedded mitochondrion, defocus 1.6 μm (Matsumoto, K., Fujita, Y., Yoneda, M., Itoh, M., Tanaka, M., Danev, R. and Nagayama, K., unpublished data). (b) modulus of the Fourier transform of (a).