Table 1. TR markers tested on Spanish strains of X. arboricola pv. pruni (n = 239), primers, PCR conditions, number of alleles and Nei’s genetic diversity (H_T).

Name	TR length (bp)	Primers	Annealing T (°C)	Primer concentration (μM)	PCR pool	Range of repeat numbers	Number of alleles (HT)a
TR51I	7	5’ FAM-CATGGCAGTGCAGGTGGATC 3’ 5’ CTGCAACTCCCGATTCCCGA 3’	68	0.3	1	4–6	3 (0.154)
TR37I	7	5’ VIC-ATGGAGGATGCGGTTGCGGCT 3’ 5’ CCAACAGAACCCCGCACCCA 3’	68	0.3	1	4–6	3 (0.384)
TR37II	6	5’PET-CGTCATGGACGCCCTGGTCAG 3’ 5’ CATTGGCATCGGCACGGCTACT 3’	68	0.3	1	9–11	3 (0.017)
TR05-06	7	5’ NED-GTCGACGGGTTCGCGGAAGGT 3’ 5’ GTGCAGCACCAGCCAAAGGCA 3’	68	0.3	1	15–21	7 (0.795)
TR40I	7	5’ FAM-TATCAGGCAGCGCACCAGCT 3’ 5’ TGGAATGTGGAGGCTGTTCG 3’	68	0.3	2	7–8	2 (0.168)
TR36I	7	5’ VIC-GCAGGAGAAGGAAAGCGCCAG 3’ 5’ CGATCGCATCTGTGTGGGTTAG 3’	68	0.3	2	5–6	2 (0.132)
TR03I	7	5’ PET-GACATTCGCCGGGAGTGCAG 3’ 5’ GGTTGCTTGGTCGTTGATCG 3’	68	0.3	2	7–8	2 (0.008)
TR68I	7	5’ NED-CTTGCGGTACTGGCTGTTCA 3’ 5’ AAATCATCGGCGCCTGAAAC 3’	68	0.3	2	9–15	7 (0.632)
TR30II	7	5’ FAM-TTCTGCCGTCTTTCAGGGCTGG 3’ 5’ CATCAGTGCGAGGCCACGAAC 3’	66	0.3	3	7–11	4 (0.371)
TR28II	9	5’ VIC-GCGGCATGTTCCGACTGCACC 3‘ 5’ GGGTGGATGAGGGTCTGCATG 3’	66	0.6	3	3–4	2 (0.008)
TR38II	6	5’ PET-TCTCGGTATCGATGTGGGTGC 3’ 5’ CCCGTAGCTGTATCAGTGCCT 3’	66	0.9	3	6–12	7 (0.691)
TR58II	7	5’ NED-GGAAGAGTACCCGGCAATTCT 3’ 5’ TCTGATCGGTGCTGAGCGTCT 3’	66	0.6	3	9–17	8 (0.667)
TR33I	7	5’ FAM-CGAGTGGATGTTATGGCGTGG 3’ 5’ CTCGCAAAACCCTTGCCATC 3’	66	0.3	4	5–8	4 (0.207)
TR58I	7	5’ VIC-ATCTGTTGCTGGCCGAGAGC 3’ 5’ ACCAACACCGAGCTTGCCTC 3’	66	0.3	4	5–9	5 (0.355)
TR79I	7	5’ NED-GCTGATCCTTCGTGGGCTTG 3’ 5’ GGTGTGAATTCGTCGGTGAC 3’	66	0.3	4	6–10	5 (0.393)
TR50I	7	5’ VIC-GTTGCGAGATCGGGCGCTTC 3’ 5’ CGTGCATCAGACGCTTGCGT 3’	68	0.3	5	5–9	5 (0.727)
TR66I	8	5’ PET-TGCAGTTGTGGTCTTCGGCA 3’ 5’ CACGCATCAAGTTCGACATGGTGC 3’	68	0.3	5	9–12	4 (0.719)
TR15I	7	5’ NED-GCCATGTCGCCGGGAAACGA 3’ 5’ TCGAGCGGTTCCTGCGGTTGT 3’	68	0.3	5	6–7	2 (0.285)
TR67II	7	5’ VIC-AGATACAAGGCGAACGCGAT 3’ 5’ CAGGACAGGAACGGCAACC 3’	64	0.3	6	10–20	11 (0.779)
Xap4790	112	5’ CGCGTATTGCAGGAATCCAC 3’ 5’ FAM-CGATTGGAGATCCGGACCAG3’	68	0.3	A	2–4	2 (0.041)
Xap4422b	116	5’ GGTCTGATCCGCTTCTCACC 5’-NED-ATCCGCGCCAACTACAAGAA	68	0.3	A
Xap0897	15	5’ ATTACTTCTTCCCCTGCGGC 3’ 5’ FAM-TCTACAACACCAAGACCGGC 3’	64	1.2	B	1–3	2 (0.008)
Xap2280	20	5’ AGAGCCTACACGGACGTACT 3’ 5’ VIC-TGAACGGGATGGTGCAAGTT 3’	64	0.3	B	7–13	6 (0.508)
TR10II	12	5’ TGGTTGCGCCCTTGCCTTCTC 3’ 5’ PET-TCGCCGGCATCAACATGGCCG 3’	64	0.9	B	5–7	3 (0.292)

^a Nei’s genetic diversity calculated using ARLEQUIN version 3.01.

^b This marker was monomorphic within Spanish strains and was only used to compare them to the worldwide collection.