Fig. 2.

Immunoblot analysis of TsAb3v1 and TsAb2v2 effect on signaling in BxPC3, H322M and MDA-MB-175-VII cells. Expression and phosphorylation status of HER1, HER3, cMet, IGF1R, AKT and MAPK in cells after a 30 minutes incubation with 0.07 µM TsAb3v1, TsAb2v2, BsAb HER1/IGF1R, BsAb cMet/HER3, and the combination of all four parental antibodies. Following the antibody incubation, cells were stimulated with the relative growth factors EGF, Heregulin, HGF and IGF1 for 10 minutes, lysed and subjected to immunoblotting. Tetra-, bi- and monospecific antibody molecules or combinations corresponding to expression and phosphorylation status of RTK are shown as ± symbols below the graph.