Table I.
Kinetic constants for binding of extracellular domains of cMet, HER3, HER1 and IGF1R receptors to TsAb3v1, TsAb2v2 and parental antibodies 5D5 anti-cMet, HER3 MAb, HER1 MAb, IGF1R MAb
| Antibody | Analyte | [M−1·s−1] | [s−1] | [min] | [M] |
|---|---|---|---|---|---|
| TsAb3v1 | cMet | 4.1E+04 ± 0.2% | 5.0E−04 ± 0.4% | 23.3 | 1.2E−08 ± 0.6% |
| TsAb2v2 | cMet | 4.1E+04 ± 0.2% | 5.4E−04 ± 0.4% | 21.6 | 1.3E−08 ± 0.5% |
| 5D5 | cMet | 6.3E+04 ± 0.2% | 2.7E−04 ± 0.4% | 43.4 | 4.2E−09± 0.5% |
| TsAb3v1 | HER3 | 8.3E+04 ± 0.2% | 7.9E−04 ± 0.1% | 14.6 | 1.0E−08 ± 0.3% |
| TsAb2v2 | HER3 | 6.9E+04 ± 0.1% | 9.3E−04 ± 0.1% | 12.5 | 1.3E−08 ± 0.2% |
| Mab HER3 | HER3 | 1.7E+05 ± 0.1% | 6.8E−04 ± 0.1% | 17.1 | 4.0E−09 ± 1.7% |
| TsAb3v1 | EGFR | 9.0E+04 ± 0.2% | 4.6E−04 ± 0.4% | 25.1 | 5.1E−09 ± 0.4% |
| TsAb2v2 | EGFR | 9.0E+04 ± 0.2% | 5.1E−04 ± 0.4% | 22.6 | 5.7E−09 ± 0.4% |
| Mab HER1 | EGFR | 8.3E+04 ± 0.2% | 6.1E−04 ± 0.3% | 18.8 | 7.4E−09 ± 0.4% |
| TsAb3v1 | IGF1R | 7.9E+05 ± 3.8% | 4.8E−03 ± 1.9% | 2.4 | 6.1E−09 ± 4.9% |
| TsAb2v2 | IGF1R | 9.4E+05 ± 3.2% | 4.5E−03 ± 1.5% | 2.6 | 4.8E−09 ± 4.2% |
| Mab IGF1R | IGF1R | 1.1E+05 ± 1.9% | 2.7E−03 ± 0.7% | 4.3 | 2.5E−09 ± 1.6% |