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. 2016 Sep 26;11(9):e0163640. doi: 10.1371/journal.pone.0163640

Fig 4. Differentiation stage-specific effects of baicalein during adipogenesis.

Fig 4

A, Addition of baicalein at the indicated time-points during adipocyte differentiation of 3T3-L1 cells for 6 days. B, Oil Red O staining of intracellular lipids in 3T3-L1 cells in DMEM with stage-specific addition of baicalein. The cells (undifferentiated cells: U; white column) were differentiated into adipocytes (differentiated cells: D) for 6 days in DMEM without (gray column) or with (50 μM; black columns) baicalein during days 0–6, 0–2, 2–4, or 4–6. C, Intracellular triglyceride level. 3T3-L1 cells (undifferentiated cells: U) were differentiated into adipocytes (differentiated cells: D) as described in the legend of Fig 3B. Data are the means ± S.D. from three experiments. **p<0.01, as indicated by the brackets.