Fig 5. Decreased expression of adipogenesis-related genes by baicalein in early stage of adipogenesis.

A, Expression levels of the adipogenic and lipogenic genes. 3T3-L1 cells (undifferentiated cells: U; white columns) were differentiated into adipocytes (differentiated cells: D) for 6 days in DMEM in the absence (gray columns) or presence (50 μM; black columns) of baicalein during days 0–2 or 0–6. Data are presented as the means ± S.D. from three experiments. **p<0.01, as indicated by the brackets. B, Decrease in glucose uptake in 3T3-L1 cells by baicalein. Cells (undifferentiated cells: U) were differentiated into adipocytes (differentiated cells: D) for 6 days in DMEM containing baicalein or not (0 or 50 μM) during days 0–2 or 0–6. Cells were further incubated with the fluorescent 2-NBDG, and then observed under a fluorescence microscope. C, Quantification of glucose uptake in baicalein-treated, differentiated 3T3-L1 cells. Cells were prepared as described in the legend of Fig 5B. The data are presented as the value relative to that of the undifferentiated cells and are shown as the means ± S.D. from three experiments. **p<0.01, as indicated by the brackets.