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. 2016 Sep 26;11(9):e0163640. doi: 10.1371/journal.pone.0163640

Fig 6. Regulation of C/EBPα-mediated expression of GLUT4 gene in baicalein-treated 3T3-L1 cells.

Fig 6

A, 3T3-L1 cells (undifferentiated cells: U; white columns) were differentiated into adipocytes (differentiated cells: D) for 6 days in DMEM without (gray columns) or with (50 μM; black columns) baicalein. The mRNA levels were quantified by qPCR. Data are presented as the means ± S.D. from three experiments. **p<0.01, as indicated by the brackets. B, ChIP assay. 3T3-L1 cells (undifferentiated cells: U) were differentiated into adipocytes (differentiated cells: D) for 6 days in DMEM without or with baicalein (0 or 50 μM) during days 0–2 or 0–6, and the ChIP assay was then performed. The resultant PCR products were analyzed by agarose-gel electrophoresis. input (input control) means that a diluted aliquot before immunoprecipitation was used for PCR amplification. The results are representative from three experiments. Band intensity was measured with MultiGauge software.