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. 2016 Sep 26;11(9):e0162730. doi: 10.1371/journal.pone.0162730

Fig 1. Identification of INCENP -TV and SNP g.19970 A>G and PCR-RFLP of of bovine INCENP gene.

Fig 1

(A) The cloning sequencing result of INCENP-cDNA by a pair of primers, F4 and R4. A new transcript INCENP -TV which deleted 12 bp was found. (B) Genomic structure of the bovine INCENP gene which consists of 20 exons, comprising 2646 bp of coding sequence. (C) The splicing pattern of the INCENP-TV splice variant which deletes exon 12, merely composed of 12 bp. (D) The SNP g.19970 A>G (rs:109416157) which lies in the region of intron 11 was discovered by sequencing (E) PCR-RFLP was employed to detect the mutation by Nde I. The products of endonuclease digestion were tested in 2.5% agarose gel, showing three genotypes: AA (606 +110 bp), AG (716 + 606 + 110 bp) and GG (716 bp).