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. 2016 Jun 17;10(4):178–186. doi: 10.1080/19336934.2016.1195938

Figure 2.

Figure 2.

Increased SCAR activity results in fusion block. A. Three hemisegments from a stage 16 embryo. Embryos are stained for F-actin (phalloidin, white) to show the muscle pattern, and YFP (GFP antibody, green) to detect YFP reconstitution. (Ai–Aii) In control embryos, UAS-myc-NYFP and UAS-HA-CYFP were expressed in muscles under the control of DMef2-Gal4. Phalloidin staining shows wild-type muscle pattern. Background fluorescent level was visualized with antibody against GFP. UAS-Abi-myc-NYFP and UAS-SCAR-HA-CYFP (Aiii–Aiv) or UAS-SCAR-myc-NYFP and UAS-Abi-HA-CYFP (Av–Avi) were expressed in muscles under the control of DMef2-Gal4. Phalloidin staining shows impaired fusion and the actin focus at the fusion site. YFP shows the localization of Abi-SCAR interaction. (B) Muscle pattern from stage 16 embryos (antibody against Myosin Heavy Chain. white). Three hemisegments are shown from each embryo. One (Bi–Biv) or 2 copies (Bv–Bviii) of split-YFP labeled Abi or SCAR were expressed in the muscles under the control of DMef2-Gal4. Abi overexpression does not change muscle pattern. Increased expression of SCAR results in myoblast fusion block in a dosage dependent manner. Scale bar: 20 μm.