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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biotechnol Bioeng. 2016 Jun 30;113(11):2328–2341. doi: 10.1002/bit.26001

Figure 1. The effect of washing and incubation conditions on enrichment ratio and recovery of yeast displaying fibronectin domain ligands panned on EGFRhigh cells.

Figure 1

Yeast displaying E6.2.6′, E6.2.6′ N78S, and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against EGFR-expressing MDA-MB-468. The enrichment and yield of binding ligands is presented as the mean ± standard deviation of 3–9 replicates. (A and B) Selections were performed under baseline conditions with the exception of varied number of washing steps. (C and D) Selections were performed with the indicated modulation of incubation conditions.