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The effects of T2, T5, and T7 on the initial rates of product formation. Each reaction mixture contained 300 μM N, 50 ± 5 μM for each of eight of nine electrophilic fragments Ei (the excluded electrophilic fragment is indicated in parentheses on the template axis), 25 ± 2 μM initial concentration of Ti (indentified on the template axis), 5 mM TCEP, 100 mM Mops (pH 7.2).
