Fig. 1.

Overview of the pipeline for metabolic profiling of wild-type and elp3Δ strains carrying indicated plasmids. The UMY4238 and UMY4239 yeast strains contained either: an empty low-copy pRS315 vector (elp3Δ-l.c.-empty); a pRS315 vector containing the wild-type ELP3 gene (elp3Δ-l.c.-ELP3); an empty high copy pRS425 vector (elp3Δ-h.c.-empty, WT-h.c.-empty); or a high copy pRS425 vector carrying the tRNA genes tK(UUU), tQ(UUG) and tE(UUC) (elp3Δ-h.c.-tKQE, WT-h.c.-tKQE). Yeast strains were cultivated logarithmically to an OD₆₀₀ value of ~0.5 at 30 or 34 °C and harvested. Metabolites were extracted and then quantified using GC-TOF-MS. Metabolite data was analyzed using multivariate analysis (PCA, PLS-DA) which separated the metabolites according to different classes representing the elp3Δ strains containing various plasmid constructs