Fig. 2.

Metabolic variation of the wild-type and elp3Δ strain containing indicated plasmids. The UMY4238 and UMY4239 yeast strains containing either: an empty low-copy pRS315 vector (elp3Δ-l.c.-empty); a pRS315 vector containing the wild-type ELP3 gene (elp3Δ-l.c.-ELP3); an empty high copy pRS425 vector (elp3Δ-h.c.-empty, WT-h.c.-empty); or a high copy pRS425 vector carrying the tRNA genes tK(UUU), tQ(UUG) and tE(UUC) (elp3Δ-h.c.-tKQE, WT-h.c.-tKQE) were grown logarithmically to an OD₆₀₀ of ~0.5 at 30 °C and harvested. Metabolites were extracted and then quantified using GC-TOF-MS. Each dot in the PCA analysis represents a technical replicate from three different biological replicates