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. 2016 Sep 27;12(12):177. doi: 10.1007/s11306-016-1120-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Score plots summarizing the PLS-DA modelling of strains grown at 34 °C. a PLS-DA score plot when modelling the elp3Δ strain with an empty low copy pRS315 vector (elp3Δ-l.c.-empty) against the elp3Δ strain containing the wild-type ELP3 gene on a pRS315 vector (elp3Δ-l.c.-ELP3). b Random permutation (20 randomizations) test-validation plot of PLS-DA model in a. c PLS-DA score plot when modelling the elp3Δ strain with an empty high copy pRS425 vector (elp3Δ-h.c.-empty) against the elp3Δ strain containing a pRS425 vector carrying the tRNA genes tK(UUU), tQ(UUG) and tE(UUC) (elp3Δ-h.c.-tKQE). d Random permutation (20 randomizations) test-validation plot of PLS-DA model in (c). Each dot in a, c and e represents a technical replicate from three different biological replicates