Fig. 5.

Metabolic alterations upon loss of ncm⁵U, mcm⁵U and mcm⁵s²U wobble uridine nucleosides in tRNA. The UMY4239 elp3Δ strains contained either: an empty pRS315 low copy vector (l.c. empty); a pRS315 vector carrying the wild-type ELP3 gene (l.c. ELP3); an empty pRS425 high copy vector (h.c. empty); or a pRS425 high copy vector carrying the tRNA genes tK(UUU); tQ(UUG) and tE(UUC) (h.c. tKQE). These yeast strains were grown logarithmically to ~0.5 OD₆₀₀ at 30 or 34 °C and harvested. Metabolites were extracted and then quantified using GC-TOF-MS. Metabolites were hierarchically organized into five clusters as represented by a–e based on metabolite abundance (see Online Resource 2). Red signifies metabolite enrichment and green signifies metabolite reduction