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. 2016 Sep 27;7:337. doi: 10.3389/fphar.2016.00337

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Copyright © 2016 Liu, Wang, Zhao, Dong, Hu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EC₅₀ of combination of DISS (3,6’-Disinapoyl sucrose) and TFSA (tenuifolisideA). Cells were treated with DISS or TFSA at desired concentrations (15.625–2,000 μM) then cell viability were determined by cell counting kit-8. DISS or TFSA response curve was generated based on the determined relative cell viability, EC₅₀ was measured. EC₅₀ of combination was tested on TFSA at a fixed concentration (40 μM) plus DISS at various concentrations (600, 300, 150, 75, 37.5, 18.75 μmol/L). The EC₅₀ of DISS, TFSA and the combination of DISS and TFSA was 606.4 ± 23.3 μM, 237.8 ± 13.3 μM, and 83.86 ± 1.06 μM, respectively. CI value of combination was 0.31 (CI < 1 means synergic effect).