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. 2016 Sep 27;7:1451. doi: 10.3389/fpls.2016.01451

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Copyright © 2016 Lonsdale, Davis, Doblin and Bacic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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After the SP region (orange) was identified using SignalP 4.1 for positive data or a fixed length of 30 amino acids for negative, five modifications were made to each original sequence in a dataset. Reverse inverted the amino acid order of the entire sequence; SP C-term placed the SP at the C-terminus, leaving the sequence length unchanged; SP Remove excluded the SP region and shortened the sequence length. SP Random and Random involved random shuffling of the SP region and the entire coding sequence, respectively. The sequences were then submitted to SecretomeP. Orange, SP region; Purple, mature protein sequence.