Figure 3.

Efficient Killing of Sau by Spn requires direct contact. Transwell chambers were installed into 6-well plates and THY was added. TIGR4 was inoculated directly in the Transwell chamber and Sau in the bottom of the well (Sp/Sau), or Sau was inoculated in the Transwell chamber and TIGR4 in the bottom (Sau/Spn). As a control Sau was inoculated alone or with TIGR4 (+Spn). Cultures were incubated for 4 h at 37°C, after which planktonic bacteria (A) or biofilms (B) were harvested from the Transwell chamber, or from the bottom of the well, serially diluted and plated onto salt mannitol agar plates. (C) Planktonic and biofilms were also plated onto BAP plates with gentamicin to obtain Spn counts. Error bars represent the standard errors of the means calculated using data from at least three independent experiments; the median (cfu/ml) is shown inside bars. ^*Statistical significance (p < 0.05) in comparison to wells inoculated with Sau.