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UV–visible spectra showing the reduction of the [2Fe-2S]1+ cluster to the all-ferrous state, [2Fe-2S]0-H+ (pH 7). The cluster remained in the [2Fe-2S]1+ state in the presence of EuII, then addition of DTPA reduced the cluster rapidly to the [2Fe-2S]0-H+ state. Experimental details are described in Materials and Methods.
