Figure 10.

Non additivity of adrenergic and cholinergic stimulation. (A) Simulating the response to 20 nM ISO. The AP firing rate increases by 13%. (B) Simulating the response to 80 nM of CCh. The AP firing rate decreases by 16%. (C) Simulating the response to 80 nM of CCh followed by the addition of 20 nM of ISO. The AP firing rate increases by 6%. (D) Simulating the response to 20 nM of ISO followed by 80 nM of CCh. The AP firing rate increases by 6%. These set of simulations show that the response to treatment by ISO and CCh is not equal to the simple addition of the individual ISO and CCh effect on the AP firing rate (13–16% = −3% ≠ 6%).