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. 2016 Sep 27;7:386. doi: 10.3389/fimmu.2016.00386

Figure 1.

Figure 1

Signaling through C40.T4 enhanced the production of cytokines and expression of CD80, CD86, and MHCII on Mtb-infected DCs. Mtb-infected DCs were stimulated through C40.T4 for 24 h. Control cultures were also set using CD40A, TLR4L, isotype-matched controls, and medium alone. Later, SNs were harvested for the detection of (A) IL-6; (B) IL-12; (C) TNF-α; (D) IL-10 by ELISA and expressed as pg/ml. Data shown as mean ± SD are normalized with their respective isotype-matched controls. CD11c+ DCs were phenotyped by flowcytometry for the expression of (E) CD80 (p < 0.001); (F) CD86 (p < 0.01); (G) MHCII. Data expressed as mean ± SD of percent positive cells are representative of three independent experiments. “*,” “**,” and “***” indicate p < 0.05, p < 0.01, and p < 0.001, respectively.