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. 2016 Sep 16;10:12–23. doi: 10.1016/j.redox.2016.09.005

Fig. 5.

Fig. 5.

IL-10 control of macrophage metabolism is via regulation of NO. Metabolic analyses were performed on WT and Il10-/- BMDM 24 h after LPS (100 ng/mL) stimulation including ECAR fold change of LPS treated BMDM compared to non-LPS treated BMDM (a), percent mitochondrial OCR (b), and OCR/ECAR ratio (c). BMDM were treated with for 24 h ±IFNγ (50 ng/mL), ±LPS (100 ng/mL), ±Pam3Csk4 (100 ng/mL) in which nitrite concentrations (d) and OCR (e) were measured. Percent mitochondrial OCR was measured after the addition of NO donor DETA/NO (1 mM) (f). WT and Nos2-/- BMDM mitochondrial OCR were measured after 24 h treatment of IFNγ±LPS (g). Nitrite concentrations were evaluated in WT and IL-10−/− BMDM 24 h post treatment ±IL-10 (50 ng/mL), ±LPS (h). Metabolic data (a–c, e–g) are representative of 2 independent experiments (mean±SEM, n=5) *p<0.05. Figs. d and h are representative figures from 3 independent experiments *p<0.05. Statistical significance for a–d, f–h were assessed by Student's t test. Statistical significance for e and g were assessed by ANOVA with a Bonferroni post-test. Error bars represent ±SEM.