Table 3.
Sensitivity and specificity obtained by the different internal 16S rRNA gene fractions, considering the complete sequence of the 16S rRNA gene. Results were calculated considering the number of large sequences classified to subspecies, species or genus as maximum result.
| Sequences | Index | Subspecies | Species | Genus |
|---|---|---|---|---|
| Unilarge | Total (Z) | 41,685 | 57,616 | 68,331 |
| V3–V5 | Sensitivity | 54.02% | 56.41% | 67.57% |
| Specificity | 74.62% | 86.34% | 89.54% | |
| True Positives | 22,520 | 32,501 | 46,173 | |
| False Positives | 7,658 | 5,140 | 5,395 | |
| V4–V5 | Sensitivity | 41.83% | 44.00% | 57.27% |
| Specificity | 64.62% | 78.86% | 83.94% | |
| True Positives | 17,436 | 25,351 | 39,135 | |
| False Positives | 9,546 | 6,795 | 7,485 | |
| V3–V4 | Sensitivity | 45.10% | 48.32% | 60.38% |
| Specificity | 66.67% | 80.13% | 85.11% | |
| True Positives | 18,801 | 27,842 | 41,255 | |
| False Positives | 9,400 | 6,902 | 7,216 |
Note: Let Z be the total number of sequences assigned by Kraken to the different taxonomic levels.