Figure 2.

Ex vivo differentiation of CD34^loCD133^lo cells. CD34^loCD133^lo cells were purified and cultured in various differentiation media. (A) CD34^loCD133^lo cells were cultured in hepatocyte differentiation medium. Representative images of cultured CD34^loCD133^lo cells immunofluorescently stained on day 0 and 21 post-culture for human ALB (green) and DAPI (blue). (B) Representative phase-contrast micrographs (left) and immunofluorescence stains for human Von Willebrand Factor (vWf) (green) and DAPI (blue) (right) are shown for endothelial cell differentiation cultures after 7 days in vitro. (C) Cells from endothelial cell differentiation cultures were stained with a CD31 antibody and analyzed by flow cytometry. The histograms of the CD31 stains of CD34^loCD133^lo cells before and after differentiation are shown. (D) Representative phase-contrast micrographs (left) and stains for Oil Red O (red) (right) are shown for adipocyte differentiation cultures after 7 days in vitro. (E) Representative phase-contrast micrographs (left) and stains for Alizarin Red S (red) (right) are shown for osteocyte differentiation cultures after14 days in vitro. The scale bar applies to all panels.