Fig. 4.

Effect of RNAi of vhp-1 on the Esp phenotype of WT (A) and sek-1(km4) (B) and mek-1(ks54) (C) mutants. Synchronized populations of L1 larvae for each strain were propagated on E. coli HT115 carrying either vector control (L4440) or Ahringer clone 44D3 (vhp-1) as described previously and transferred to lawns of P. aeruginosa strain PA14 at the L4 larval stage. At least 40 worms were scored for each assay and scored as dead when they no longer responded to touch. Representative plots of multiple experiments are shown.