Figure S1.

Scheme of 3D Classification of Data, Related to Figure 1

(A) For Sample1 (without IF2) 666,610 particles were selected after 2D classification and an initial 3D refinement was done. The ‘ligands mask’ used for focused 3D classification is shown by an outline. The scheme shows how each PIC was obtained. (See ‘Image processing and structure determination’ in Methods Details for detailed information).

(B) For Sample2 (with IF2) 803,433 particles were selected after 2D classification and an initial 3D refinement was done. The ‘IF2 mask’ as well as ‘ligands mask’ used for focused 3D classification is shown by an outline. The scheme shows how PICs- I to III were obtained. (See ‘Image processing and structure determination’ in Methods Details for detailed information).