REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
IF1 Thermus thermophilus	Carter et al., 2001	N/A
IF2 Thermus thermophilus	This paper	N/A
IF3 Thermus thermophilus	This paper	N/A
fMet-tRNA Escherichia coli	Schmitt et al., 1998	N/A
Deposited Data
EMD-4073	Electron Microscopy Data Bank (EMDB)	http://www.emdatabank.org/
EMD-4074	EMDB	http://www.emdatabank.org/
EMD-4075	EMDB	http://www.emdatabank.org/
EMD-4076	EMDB	http://www.emdatabank.org/
EMD-4077	EMDB	http://www.emdatabank.org/
EMD-4078	EMDB	http://www.emdatabank.org/
EMD-4079	EMDB	http://www.emdatabank.org/
EMD-4080	EMDB	http://www.emdatabank.org/
EMD-4081	EMDB	http://www.emdatabank.org/
EMD-4082	EMDB	http://www.emdatabank.org/
EMD-4083	EMDB	http://www.emdatabank.org/
5LMN	Protein Data Bank (PDB)	http://www.wwpdb.org/
5LMO	PDB	http://www.wwpdb.org/
5LMP	PDB	http://www.wwpdb.org/
5LMQ	PDB	http://www.wwpdb.org/
5LMR	PDB	http://www.wwpdb.org/
5LMS	PDB	http://www.wwpdb.org/
5LMT	PDB	http://www.wwpdb.org/
5LMU	PDB	http://www.wwpdb.org/
5LMV	PDB	http://www.wwpdb.org/
Experimental Models: Organisms/Strains
Escherichia coli BL21(DE3)	Invitrogen	Cat#C600003
Thermus thermophilus		N/A
Escherichia coli K12		N/A
Recombinant DNA
${\text{pBStRNA}}_Y^{f\text{Met}}$	Schmitt et al., 1998	N/A
pET13a	Gerchman et al., 1994	N/A
pET30a	Merck Millipore, Novagen	Cat#69909
Sequence-Based Reagents
mRNA sequence: 5′GCUCUUUUAACAAUUUAUCAGGCAAGGAGGUAAAAAUGUUCA-3′	Integrated DNA Technologies	N/A
Software and Algorithms
MotionCorr	Li et al., 2013	http://cryoem.ucsf.edu/software/driftcorr.html
CTFFIND3	Mindell and Grigorieff, 2003	http://grigoriefflab.janelia.org/ctf
Relion-1.4	Scheres, 2012	http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
Eman2	Tang et al., 2007	http://blake.bcm.edu/emanwiki/EMAN2
Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
Coot v0.8	Emsley et al., 2010	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Refmac v5.8	Brown et al., 2015	https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/refmac/refmac.html
Molprobity	Chen et al., 2010	http://molprobity.biochem.duke.edu/
Pymol	DeLano, 2006	http://www.pymol.org