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. 2016 Oct;148(4):341–355. doi: 10.1085/jgp.201611604

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© 2016 Tsukamoto et al.

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Figure 3. — Comparison of a time course of CaT detected by α-actinin–YC-Nano140 with that by Fluo-4 in cardiomyocytes at different temperatures during spontaneous beating. (A) Time course of a change in F_yellow/F_cyan (α-actinin–YC-Nano140) or Fluo-4 fluorescence in cardiomyocytes at 22°C and 37°C. CaT was normalized at the peak for all curves. Each curve was averaged from five beats obtained in 8–24 cells (see B for experimental numbers). (B) Graph comparing the time to peak values for α-actinin–YC-Nano140 or Fluo-4 at 22°C and 37°C. n = 57 beats (8 cells), 61 beats (8 cells), 514 beats (24 cells), and 203 beats (14 cells) for Fluo-4 (22°C), α-actinin–YC-Nano140 (22°C), Fluo-4 (37°C), and α-actinin–YC-Nano140 (37°C), respectively. (C) Graph comparing the decay time values for α-actinin–YC-Nano140 and Fluo-4 at 22°C and 37°C. n, same as in B. (B and C) ***, P < 0.001 (Tukey-Kramer test). Bars in each graph indicate mean values.