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. 2016 Sep 27;6:33787. doi: 10.1038/srep33787

Figure 7. PI3K inhibitor LY294002 represses As2O3-induced EndMT in HAECs.

Figure 7

(a) Western blotting results for relative protein levels of VE-cad, CD31, α-SMA and FSP1 in different groups. +LY294002 indicates the co-application of LY294002 and As2O3 (8 μmol/l). (b) Relative mRNA expression levels of VE-cad, CD31, α-SMA, FSP1, FN and Vimentin in each group by qRT-PCR analysis. (c) Effects of LY294002 on the mRNA expression of fibrosis-related genes Col1a, Col3a, mmp2, mmp9. *p < 0.05, **p < 0.01 vs. Control. #p < 0.05, ##p < 0.01 vs. As2O3. Data are shown as mean ± SEM, n = 3–5. (d) Immunofluorescence analysis for the expression levels of CD31 and α-SMA in Control, LY294002, As2O3 and As2O3 + LY294002 groups. Scale bar = 30 μm.