Skip to main content
. 2016 Sep 27;6:33916. doi: 10.1038/srep33916

Figure 1. HB-GAM turns the CSPG aggrecan to a potent activator of neurite growth.

Figure 1

(a–c) Neurite outgrowth in cortical neurons on substrates coated with aggrecan (AGG), aggrecan + IgG or aggrecan + HB-GAM. Neurons display poor attachment and no neurite growth on aggrecan (10 μg/ml) or aggrecan + IgG (both at 10 μg/ml)-precoated substrate. (c,d) In contrast, HB-GAM precoated together with aggrecan (both at 10 μg/ml) induces neurite growth (150–200 μm/neuron) compared to unprecoated plastic, aggrecan or aggrecan + IgG. (c,e) HB-GAM (10 μg/ml) added to the culture medium induces robust neurite outgrowth on the substrate coated with aggrecan but even inhibits neurite outgrowth on uncoated substrate. The scale bar in a (20 μm) is valid for (a,b,d,e). Error bars represent standard error of mean (SEM), symbols *, ** and *** represent P < 0.05, 0.01 and 0.001, respectively. All data are based on 3 independent experiments.