Figure 4. HB-GAM prevents PTPσ binding to chondroitin sulfates of aggrecan.

Extracellular domain of wild type and mutant PTPσ (80 nM) was mixed with different concentrations of HB-GAM. The mutant PTPσ lacks the CS binding site. The mixture was added to wells coated with aggrecan (10 μg/ml) and bound PTPσ was measured with HPR conjugated goat anti-human IgG (FC part) using the O-phenylendiamine dihydrochloride colorimetric assay.