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. 2016 Sep 27;6:33955. doi: 10.1038/srep33955

Figure 5. FISH analysis of meiosis in both the wild-type and the TE5A mutant.

Figure 5

Meiotic chromosomes probed with 45S rDNA (green). Chromosomes were stained with DAPI (blue). (A,E), at leptotene stage, 12 to 14 45S rDNA signals were observed in the wild-type (A) and in the TE5A mutant (E), respectively; (B,F), at pachytene stage, six paired signals (half of the total number) were detected in the wild type (B), whereas 12 to 14 45S rDNA signals were still observed in the mutant (F,C,D,G) signals in half were observed in dyads (C) and tetrads (D) of the wild-type, in contrast, the number of 45S rDNA FISH foci was not halved, and unpaired 45S rDNA FISH foci were still observed in the mutant (G). Scale bars = 5 μm.