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. 2016 Sep 27;6:33843. doi: 10.1038/srep33843

Figure 6. Identification of broadly neutralizing antibody (bnAb) and H7N9 specific antibody in Ig repertoires of H7N9 infected patients.

Figure 6

(a) Alignment of previously reported bnAb sequence (FI390) and the antibody sequence found in H7N9 patient. FI390 was as a bnAb against influenza virus reported by Pappas L. N2_3050 was the IGH sequence found in two recovered H7N9 patients with identical CDR3 amino acid sequence as FI390. Alignment was performed using Clustal W2. (b) Binding kinetics of scFvs N2_3050, o1b1 and l1b1 to H7N9 HA antigen using Surface Plasmon Resonance assays. Different concentrations (10 uM, 2 uM, 400 nM, 200 nM, 100 nM and 50 nM for N2_3050 and c1b1; 1 uM, 500 nM, 250 nM, 125 nM, 62.5 nM and 31.5 nM for l1b1, respectively) is used in the test. Calculated KD values are shown in the table presenting binding affinity of each scFvs.