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TSLP does not support the growth of BM B cell precursors from adult λ5° mice. Shown is a limiting dilution analysis of pro-B cells, isolated from adult BM of λ5° (▴), BALB/c.Rag2° (○), and BALB/c mice (•) in the presence of TSLP. The best-fit line crossing the origin is plotted. The dashed line intercepts the vertical axis at 37%. Data are representative of two experiments. No growth was observed in cultures not supplemented with either IL-7 or TSLP (not shown).
