Table 2. Frequency of BM B cell precursors responding to soluble factors.
| Factors
|
|||
|---|---|---|---|
| Mice | BM population | IL-7 | TSLP |
| C57BL/6 | CD19+CD43+ | ND | 1/12; 1/10 |
| H3 × Rag2° | CD19+CD43+ | 1/30; 1/33 | 1/60; 1/25; 1/31 |
| Rag2° | CD19+CD43+ | 1/60 | <1/5,000; <1/473 |
The indicated cell populations were purified by cell sorting from the BM of adult C57BL/6, H3 × Rag2°, or Rag2° mice. Cells were plated into wells containing irradiated NIH-3T3 feeder cells with medium supplemented with either IL-7 or TSLP. The growth of colonies was scored 7 days later. The multiple results shown per genotype were obtained in different experiments. ND, not determined.