Figure 6. One to three-minute time intervals can describe the robust CMPI utilizing various image acquisition systems.

(a) CMPI of 6 different cell types calculated at 1, 2, 3, 5, or 10 min time intervals are compared against the corresponding diffusion coefficients, μ. (b) Random Gaussian noise-implemented CMPI values are plotted against the standard deviation of the Gaussian noise (ranging from 0–1.0 μm). The initial data were obtained from NIH 3T3 fibroblasts at 1-min time intervals. Inset: The distribution of system noise was determined from the centroid positions of a fixed cell. (c) The fitting results (R²) between noise-implemented CMPI and corresponding diffusion coefficients, μ, of 6 cell types were plotted against the standard deviation of introduced noise (ranging from 0–1.0 μm). CMPI were calculated using data extracted at 1-, 2-, 3-, 5-, and 10-min intervals from 1-hour movies of the 6 different cell types.