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. 2016 Sep 27;6:34125. doi: 10.1038/srep34125

Figure 1. JMJD8 positively regulates NF-κB.

Figure 1

(a) HEK293T cells transfected with control and JMJD8 targeting siRNA oligos were treated with and without 10 ng/ml of TNFα for 0, 0.5, 2, 6 and 12 hours. The expression of TNFα, IL8, CCL5 and IκBα were measured by RT-qPCR (n = 4). (b) HEK293T cells transfected with control, JMJD8a and JMJD8b siRNA oligos were treated with and without 10 ng/ml of TNFα for 2 hours, the expression of TNFα and IL8 were measured by RT-qPCR. The knockdown expression of JMJD8 by each siRNA oligo was verified by immunobloting with a JMJD8 specific antibody (n = 4). (c) JMJD8 knockdown HEK293T cells reconstituted with JMJD8 were treated with TNFα for 2 hours and the expression of TNFα and IL8 were measured by RT-qPCR. The transient expression of ectopic JMJD8 was verified by immunobloting with a JMJD8 specific antibody (n = 4). (d) Control and JMJD8 knockdown HEK293T cells were infected with Sendai virus (150 HAU/ml) and the levels of IFNβ were measured by RT-qPCR (n = 4). Data represent the means ± SD. (*p > 0.05, **p > 0.01). Full-length blots are presented in Supplementary Fig. S3.