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. 2016 Sep 27;6:34125. doi: 10.1038/srep34125

Figure 3. JMJD8 is required for TNF-induced IKK kinase activity.

Figure 3

(a) Control and JMJD8 knockdown HEK293T cells were induced with 10 ng/ml of TNFα for 0, 5, 10, 15 and 30 minutes. IKK kinase activity was measured with an in vitro kinase assay followed by immunoblotting using the anti-p-IκBα and anti-IKKβ antibodies. Relative intensity of bands were quantified using the Image Lab (BioRad)/ImageJ, were normalized to IKKβ, and shown in relative to 0 minute of siControl (n = 2). (b) Control and JMJD8 knockdown HEK293T cells were induced with 10 ng/ml of TNFα for 0, 15, 30, 45 and 90 minutes. Total cell lysates were prepared and immunoblotted with the indicated antibodies (n = 2). Data represent means ± SD. (*p > 0.05). Full-length blots are presented in Supplementary Figs S5–S8, respectively.