Table 1. Total protein yields of wild type EGFR-eYFP (WT) and full-length suppression product (Amb) obtained using the corresponding standard as well as the IRES template in the absence and presence of poly G, respectively.
| Template | Fraction | IRES | Poly G | Total protein [μg/ml] |
|---|---|---|---|---|
| WT | Supernatant fraction | − | − | 2.10 ± 0.16 |
| + | − | 5.65 ± 0.15 | ||
| + | + | 12.84 ± 0.68 | ||
| Microsomal fraction | − | − | 2.37 ± 0.60 | |
| + | − | 6.28 ± 0.27 | ||
| + | + | 8.04 ± 1.30 | ||
| Amb | Supernatant fraction | − | − | 0.51 ± 0.05a |
| + | − | 0.34 ± 0.04a | ||
| + | + | 4.47 ± 0.40a | ||
| Microsomal fraction | − | − | 0.07 ± 0.02a | |
| + | − | 1.29 ± 0.16a | ||
| + | + | 2.66 ± 0.50a |
Errors represent the standard deviation of triplicate analysis.
aTotal protein yields were estimated from the eYFP fluorescence of the suppression product in relation to the calculated total yields and eYFP fluorescence of the wild type protein synthesized under identical conditions.