Table 2. FRET efficiencies measured by flow cytometry.

Labeled epitopes	Donor mAb	Acceptor mAb	E ± SD, %
IL-15Rα and IL-2Rα	Cy3-anti-FLAG	Cy5-anti-Tac	27.5 ± 1.0
	Cy3-anti-FLAG	Cy5-7G7 B6	19.2 ± 2.1
	Cy3-7A4 24	Cy5-anti-Tac	24.4 ± 2.8
	Cy3-7A4 24	Cy5-7G7 B6	15.9 ± 3.0
IL-15Rα and IL-15Rα	Cy3-7A4 24	Cy5-7A4 24	22.6 ± 2.8
IL-15Rα and MHC I	Cy3-7A4 24	Cy5-W6/32	33.4 ± 3.1
IL-15Rα and β2m	Cy3-7A4 24	Cy5-L368	34.4 ± 3.0
IL-15Rα and MHC II	Cy3-7A4 24	Cy5-L243	56.6 ± 6.1
IL-2/15Rβ and IL-15Rα	Cy3-Mikβ3	Cy5-anti-FLAG	13.2 ± 4.0
	Cy3-Mikβ3	Cy5-7A4 24	4.7 ± 2.2
γc and IL-15Rα	Alexa 546-TUGH4	Cy5-7A4 24	10.6 ± 1.8
	Alexa 546-TUGH4	Cy5-anti-FLAG	14.6 ± 1.3
IL-2Rα and MHC I	Cy3-anti-Tac	Cy5-W6/32	30.5 ± 1.1
IL-2Rα and MHC II	Cy3-anti-Tac	Cy5-L243	44.9 ± 0.3

Data represent mean ± SD values of at least three measurements.