Figure 5. Injury with 2 mm stage displacement alters synaptic density.

(A) Representative image of uninjured iCell neurons stained with MAP2, Synaptophysin, and Hoechst 33342. Synaptophysin staining was punctate and distributed across soma and along neurites. (B) Injury did not change the ratio of synaptophysin positive cell area to total cell area (n = 12). (C) Injury did not significantly change the ratio of synaptophysin positive neurite area to total neurite area, although there was a modest downward trend (n = 12). (D) Injury significantly increased the ratio of synaptophysin positive soma area to total soma area (n = 12, *=t-test with significance criterion Bonferroni corrected to p < 0.05/3, bars = standard error).