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. 2016 Sep 27;6:34097. doi: 10.1038/srep34097

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(A) Neurons injured with 38% strain and imaged 4 hours post injury with calcein AM (green) and Hoechst 33342 staining (blue) (B) Automated segmentation of the image into cell bodies and neurites. Note that beads on neurites are rejected as cell bodies based on their size and the absence of Hoechst-positive nuclei. Extracellular nuclei are rejected as cell bodies based on the absence of calcein AM staining.