Table 1.
Localization and evaluation of amino acid (AA) differences between ELC18 framework regions (FR), those coded by ELC18 most similar human germline genes, and Germinality Index (GI) of different FRs.
| Framework Region | Total Number of AAs | Number of Identical AAs | Number of Very Similar AAs | Number of Similar AAs | Number of Dissimilar AAs | Number of Very Dissimilar AAs | |
|---|---|---|---|---|---|---|---|
| VH | |||||||
| FR1-IMGT | 25 | 20 | 0 | 2 | 2 | 1 | |
| FR2-IMGT | 17 | 16 | 0 | 0 | 1 | 0 | |
| FR3-IMGT | 38 | 33 | 0 | 2 | 2 | 1 | |
| FR4-IMGT | 11 | 9 | 0 | 0 | 1 | 1 | |
| FR-IMGT | 91 | 78 | 0 | 4 | 6 | 3 | |
| GI | 87.5% | 100% | 0% | 4.4% | 6.6% | 3.3% | |
| VL | |||||||
| FR1-IMGT | 26 | 23 | 1 | 1 | 0 | 1 | |
| FR2-IMGT | 17 | 15 | 1 | 0 | 1 | 0 | |
| FR3-IMGT | 36 | 33 | 0 | 0 | 2 | 1 | |
| FR4-IMGT | 10 | 9 | 0 | 1 | 0 | 0 | |
| FR-IMGT | 89 | 80 | 2 | 2 | 3 | 2 | |
| GI | 89.9% | 100% | 2.2% | 2.2% | 3.4% | 2.2% | |
| VH and VL | 180 | 158 | 2 | 6 | 9 | 5 | |
| GI | 87.8% | 100% | 1.1% | 3.3% | 5.0% | 2.8% | |