Figure 4.

Changes in 3-epi-DON concentrations in E. coli BL21(DE3) cells overexpressing Fusarium graminearum Tri101. (a) No changes in 3-epi-DON recoveries were noticed for the construct that encoded the Tri101 enzyme in comparison to the control or empty vector. The above tendency was observed in two separate experiments, each with three replications, and the different letters signify significant differences between the means according to Fisher’s Least Significant Difference (LSD) test (p < 0.005); (b) To confirm that the above cells where all over-expressing His-FgTri101, bacterial pellets were collected, lysed, and ran on SDS-PAGE gels (4%–20%) before Coomassie Blue staining. The overexpression levels of His-FgTri101 were all evident in both DON and 3-epi-DON treatments.