Fig. 2.

enJS56A1 assembles intracytoplasmic viral particles. 293T cells were transfected with JSRV (A–D) or enJS56A1 (E and F) or cotransfected (G and H) with both expression plasmids and analyzed by electron microscopy. (A and B) Extracellular complete JSRV particles and particles approaching and budding from the membrane. (C and D) Intracytoplasmic JSRV. (E and F) enJS56A1 particles. (C and F) Arrows, incomplete viral particles. In E, arrows point to apparent projections from the enJS56A1 particles. (G and H) Large clusters of perinuclear viral particles in enJS56A1+JSRV cotransfected cells. N, nucleus.