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. 2016 Sep 12;8(9):267. doi: 10.3390/toxins8090267

Figure 2.

Figure 2

The circular dichroism spectra and CYP3A29 and the mutants’ nifedipine oxidation activities. (A) Far-UV CD spectra of CYP3A29 and its mutants. CD spectra were recorded at 20 °C and in 50 mM potassium phosphate buffer (200 mM KCl, 0.2 mM DTT, 1 mM EDTA, and 20% glycerol, pH 7.4). (B–G) The HPLC chromatogram of oxidized nifedipine standard; the HPLC chromatogram of nifedipine standard; HPLC chromatograms of nifedipine metabolized by WT, S119A, F304A, and T309A, respectively. HPLC eluent was monitored by absorbance at 254 nm.