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. 2016 Sep 25;64(10):623–636. doi: 10.1369/0022155416665098

Figure 1.

Figure 1.

MMP20 and DSPP co-localize in normal human and monkey kidney tissue. IHC on normal human kidney sections showed immunoreactivity of MMP20 (A, red, alkaline phosphatase chromogen), DSPP (B, black, HRP chromogen), and their co-localization (C, reddish black) in proximal, distal, and collecting tubules. Similar to human kidneys, IHC on monkey kidney sections showed immunoreactivity of MMP20 (D, red, alkaline phosphatase chromogen), DSPP (E, brown, HRP chromogen), and their co-localization (F, reddish brown) in proximal, distal, and collecting tubules. Double-labeled IHC of OSCC served as positive (G, reddish black; MMP20, red alkaline phosphatase chromogen; and DSPP, black HRP chromogen) control. Normal human oral mucosa served as tissue negative control (H), whereas preimmune IgG negative control on monkey sections is shown in panel I. Abbreviations: MMP20, matrix metalloproteinase 20; DSPP, dentin sialophosphoprotein; OSCC, oral squamous cell carcinoma. Scale bars = 100 µm.